We selected different concentrations of ethephon, to stress C. roseus. We used qRT-PCR and HPLC followed by PCA to obtain\ncomprehensive profiling of the vinblastine biosynthesis in response to ethephon. Based on our findings, the results showed that the\nhigh concentration of ethephon had a positive effect at both transcriptional and metabolite level.Meanwhile, there was a remarkable\ndecrease of hydrogen peroxide content and a promoted peroxidase activity in leaves. The loading plot combination with correlation\nanalysis suggested that CrPrx1 could be regarded as a positive regulator and interacts with ethylene response factor (ERF) to play a\nkey role in vinblastine content and peroxidase (POD) activity. This study provides the foundation for a better understanding of the\nregulation and accumulation of vinblastine in response to ethephon.
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